Born and raised in Berlin. 2001-2005 she studied Acting at the University Mozarteum in Salzburg, Austria, and had guest appearances at Salzburger Festspiele and Stadttheater Fürth.
2005 she got engaged to Gerhart-Hauptmann-Theater Zittau and afterwards from 2009 to 2012 worked as a freelancer in Berlin. Guest Engagements at Theater Plauen-Zwickau, Musiktheater Görlitz, Pumpenhaus Münster, GHT Zittau and TdJW Leipzig.
Since the season 2012/13 Anna-Lena Zühlke is an ensemble member at Theater der Jungen Welt. Besides her work as an actress she is since 2007 regularly working as a speaker and did camera acting for several advertisement campaigns.